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GITR Antibody
SKU: orb757404
Featured
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC |
|---|---|
| Dilution range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM79 |
| Target | GITR |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Disclaimer | For research use only |
Alternative Names
−AITR, GITR, TNFRSF18, CD357
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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human GITR protein, hFc-His tagged protein orb689379 can bind Rabbit anti-GITR monoclonal antibody (clone: DM79) in a linear range of 1-100 ng/ml.
Expi 293 cell line transfected with irrelevant protein (A) and human GITR (B) were surface stained with Rabbit anti-GITR monoclonal antibody 1 μg/ml (clone: DM79) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Flow cytometry data of serially titrated Rabbit anti-GITR monoclonal antibody (clone: DM79) on PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit anti-GITR mAb clones by titration of different concentration onto PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Flow cytometry analysis of antigen binding of rabbit anti-human GITR mAb (orb757404). (A) orb757404 does not bind to 293T cells that do not express GITR. (B) A clear peak shift of orb757404 was seen compared to the control when incubated with GITR-expressing 8226 cells, indicating strong binding of orb757404 to GITR. Antibodies were incubated at 2 μg/mL.
Quick Database Links
Gene Symbol
GITR
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
GITR Antibody (orb757404)
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