CD47 Antibody / IAP / Integrin Associated Protein

SKU: orb749906

Description

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Images & Validation

−

Item 1 of 4

Tested Applications	FACS, IF
Dilution range	Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
Reactivity	Human
Application Notes	Optimal dilution of the HCAM antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	IAP/964
Immunogen	Recombinant human protein was used as the immunogen for the CD47 antibody.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

CD47 Antibody / IAP / Integrin Associated Protein

Immunofluorescent staining of live human Jurkat cells with CD47 antibody (green, clone IAP/964) and Reddot nuclear stain (red).

Immunofluorescent staining of PFA-fixed human MCF7 cells with CD47 antibody (green, clone IAP/964) and Reddot nuclear stain (red).

Flow cytometry staining of live human Jukat cells with CD47 antibody (clone IAP/964); Red = isotype control, Blue = CD47 antibody.

SDS-PAGE analysis of purified, BSA-free CD47 antibody (clone IAP/964) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q08722

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

View Protocol

Immunofluorescence

View Protocol

Find More Protocols