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Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Microbiology, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF: 1:25, WB: 1:1000, WB: 1:1000, WB: 1:500 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-146 amino acids from human Cleaved LC3A. Antigen Region: 110~146. |
| Target | MAP1LC3A |
| Molecular Weight | 14272 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
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Western blot analysis of lysates from A431, Hela cell line, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A) (upper) or Beta-actin (lower).
Western blot analysis of lysates from A431, Hela cell line, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A) (upper) or Beta-actin (lower).
Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) Antibody (upper) or Beta-actin (lower).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse mouse embryonic fibroblasts cell line) cells labeling Pdx1 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). The nuclear counter stain is DAPI (blue). Immunofluorescence image showing cytoplasm on NIH/3T3 cell line.
Quick Database Links
Gene Symbol
MAP1LC3A
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
Cleaved LC3A Antibody (orb1787989)
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