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Description
Images & Validation
−| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD35946 |
| Immunogen | This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-429 amino acids from human EIF4B. Antigen Region: 400-429 aa. |
| Target | EIF4B |
| Molecular Weight | 69151 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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25 μl, 200 μl, 100 μl, 50 μlPhospho-EIF4B (Ser422) Rabbit Polyclonal Antibody (RBITC) [orb1045060]
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4B-S422, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (S422). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4B gene (Lane 2).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001408.2 |
|---|
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Protocol Information
EIF4B Antibody (S422) (orb1939531)
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