Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human EpCAM (101-200/314aa)
Target	EPCAM
Molecular Weight	35 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Adenocarcinoma associated antigen; CD326; CD326 antigen; Cell surface glycoprotein Trop 1; CO17 1A; EGP; EGP40; Ep CAM; Epithelial cell surface antigen; Epithelial cellular adhesion molecule; Epithelial glycoprotein; GA733 2; hEGP 2; KS 1/4 antigen; KSA; Lymphocyte antigen 74; M1S2; M4S1; Major gastrointestinal tumor associated protein GA733 2; MIC18; MK 1; TACD1; TACSTD1; TROP1; Tumor associated calcium signal transducer 1.

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Blank control: A431. Primary Antibody (green line): Rabbit Anti-TEpCAM antibody (orb183277), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 3 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Large intestine (Mouse) Lysate at 40 ug, Lane 2: Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-EpCAM (orb183277) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 38 kD.

Quick Database Links

Gene Symbol

EPCAM

UniProt

P16422

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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