Human Free Fatty Acid Receptor 4 (FFAR4) ELISA Kit

SKU: orb778084

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Free Fatty Acid Receptor 4(FFAR4).

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FFAR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FFAR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FFAR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FFAR4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.63-40 ng/mL
Sensitivity	0.254 ng/mL
Concentration	40 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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GPR129 ELISA Kit, GT01 ELISA Kit, PGR4 ELISA Kit, GPR120 ELISA Kit, O3FAR1 ELISA Kit, G Protein Coupled Receptor 120 ELISA Kit, Omega-3 fatty acid receptor 1 ELISA Kit

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Human Free Fatty Acid Receptor 4 (FFAR4) ELISA Kit

Quick Database Links

UniProt

Q5NUL3

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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