Human Melanoma Associated ME20 (ME20M) ELISA Kit

SKU: orb775750

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Associated ME20(ME20M).

Research Area

Cancer Biology, Immunology & Inflammation, Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ME20M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ME20M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ME20M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ME20M in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.056 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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PMEL ELISA Kit, GP100 ELISA Kit, SI ELISA Kit, SIL ELISA Kit, SILV ELISA Kit, Pmel17 ELISA Kit, Silver Homolog ELISA Kit, Premelanosome Protein ELISA Kit, Silver Locus Protein Homolog ELISA Kit, 95 kDa melanocyte-specific secreted glycoprotein ELISA Kit

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Human Melanoma Associated ME20 (ME20M) ELISA Kit

Quick Database Links

UniProt

P40967

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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