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Human TNF a Protein (His Tag)
SKU: orb169442
Description
Images & Validation
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| Application Notes |
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Key Properties
−| Source | Escherichia Coli |
|---|---|
| Biological Activity | The ED50 was determined in the presence of actinomycin D by a cytotoxicity assay using murine L929 cells is 2.0 × 107IU/mg. |
| Protein Sequence | MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL |
| Purity | Greater than 97.0% as determined by SDS-PAGE and HPLC analyses. |
Storage & Handling
−| Storage | Stability: Lyophilized TNF-α although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-α should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
| Disclaimer | For research use only |
Alternative Names
−TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
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Human TNF a Protein (His Tag) (orb169442)
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