PU.1 Antibody / SPI1

SKU: orb1151709

Description

Mouse monoclonal antibody to PU.1

Images & Validation

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Item 1 of 5

Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the PU.1 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	PU1/2118
Immunogen	A portion of amino acids 16-170 was used as the immunogen for the PU.1 antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human colon carcinoma tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Hodgkins Lymphoma tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Lymph Node tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PU.1 antibody (clone PU1/2118) as confirmation of integrity and purity.

Quick Database Links

UniProt

P17947

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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