Rat Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kit

SKU: orb780472

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 1A(TNFRSF1A).

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFRSF1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFRSF1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFRSF1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFRSF1A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.058 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD120A ELISA Kit, P55 ELISA Kit, TBP1 ELISA Kit, FPF ELISA Kit, TNF-R ELISA Kit, TNF-R-I ELISA Kit, TNF-R55 ELISA Kit, TNFAR ELISA Kit, TNFR1 ELISA Kit, TNFR55 ELISA Kit, TNFR60 ELISA Kit, P55-R ELISA Kit, P60 ELISA Kit, Tumor necrosis factor receptor 1 ELISA Kit, Tumor necrosis factor-binding protein 1 ELISA Kit

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Rat Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) ELISA Kit

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UniProt

P22934

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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