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SARS-CoV-2 (COVID-19) nucleocapsid Rabbit Polyclonal Antibody

SKU: orb1294283

Description

Rabbit polyclonal antibody to SARS-CoV 2 nucleocapsid protein

Research Area

Infectious Disease & Virology

Images & Validation

Tested ApplicationsIF, NeA, WB
Dilution RangeWestern Blot 1:3000-1:5000, Immunofluorescence 1:500-1:1000
ReactivityVirus

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit-IgG
ImmunogenRecombinant fragment corresponding to SARS-CoV 2 nucleocapsid protein
TargetSARS-CoV-2 (COVID-19) nucleocapsid
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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SARS-CoV-2 (COVID-19) nucleocapsid Rabbit Polyclonal Antibody

All lanes: anti-SARS-CoV-2 nucleocapsid protein antibody at 1/3000 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294283 overnight at 4°C.

SARS-CoV-2 (COVID-19) nucleocapsid Rabbit Polyclonal Antibody

Immunofluorescence: Cells were stained with orb1294283 anti-SARS-CoV-2 nucleocapsid protein antibody (green) at 1:500 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IF
Immunofluorescence
View Protocol

SARS-CoV-2 (COVID-19) nucleocapsid Rabbit Polyclonal Antibody (orb1294283)

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25 μl
¥ 3,380.00
100 μl
¥ 5,070.00
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