AKT3 Rabbit Polyclonal Antibody

SKU: orb6787

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Description

Research Area

Akt/PKB Signaling Pathway, Apoptosis, Cell Cycle, Metabolism, Nuclear Signaling Pathway, PI3K/AKT Signaling Pathway

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Canine, Equine, Gallus, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human AKT3 (101-200/479aa)
Target	AKT3
Molecular Weight	56 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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AKT3 kinase; DKFZP434N0250; PKB gamma; PKBG; PRKBG; Protein Kinase AKT3; Protein Kinase B gamma; RAC gamma; RAC gamma Serine/Threonine Protein Kinase; RACPK gamma; Serine threonine protein kinase Akt 3; Serine Threonine Protein Kinase AKT3; STK 2; STK2; V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma; VAKT Murine Thymoma Viral Oncogene Homolog 3; AKT3_HUMAN.

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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AKT3) Polyclonal Antibody, Unconjugated (orb6787) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Testis (Mouse) Lysate at 40 ug, Primary: Anti-AKT3 (orb6787) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 60 kD.

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human SH-SY5Y cell lysates, Primary: Anti-AKT3 (orb6787) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 60 kDa.

Quick Database Links

Gene Symbol

AKT3

UniProt

Q9Y243

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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