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Polyclonal antibody production involves immunising an animal with a specific antigen, which triggers the animal's immune system to produce a variety of antibodies targeting different epitopes on the antigen. These antibodies are then harvested from the animal's serum or plasma. Since multiple B cells are involved in producing antibodies, the resulting antibody mixture is referred to as "polyclonal." These antibodies recognise various epitopes on the antigen, leading to broad binding specificity.
Biorbyt provides a reliable and diverse range of custom polyclonal antibody production services, offering multiple options for immunogens and host species to accommodate various research requirements.
Advantages of Biorbyt's Polyclonal Antibody Products
Broad Binding Specificity
Polyclonal antibodies recognise multiple epitopes on the target antigen, providing broader binding specificity compared to monoclonal antibodies.
High sensitivity
Due to their ability to bind to multiple epitopes, polyclonal antibodies often exhibit higher sensitivity in assays, enabling detection of low-abundance targets.
Availability
Polyclonal antibodies can be generated relatively quickly and cost-effectively.
Versatility
Polyclonal antibodies are useful for detecting various forms and isoforms of target proteins, making them versatile tools for research, diagnostics, and therapeutic applications.
Natural Immune Response
Polyclonal antibodies mimic the natural immune response, providing a diverse repertoire of antibodies similar to those produced in vivo.
Cross-Reactivity
Polyclonal antibodies may exhibit cross-reactivity with related antigens, allowing for the detection of closely related targets
Service Process
Peptide
Lead Time: 12-15 weeks
Recombinant Protein
Lead Time: 15-17 weeks
Native Protein
Lead Time: 12-15 weeks
Process
- Antigen preparation
- The animals are immunised
- Serum titer is detected
- Affinity or protein A/G purification
- WB validation with antigen
Deliverables
- QC report of antigen
- ELISA titer guarantee 1:10000
- WB guarantee for antigen
- 1 ml of preimmune serum. 2 ml of antiserum
- Antibody purity guarantee 90% by SDS-PAGE detection
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