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Caspase-3 Recombinant Rabbit Monoclonal Antibody
SKU: orb2563024
Featured
Description
Research Area
Apoptosis, Caspases, Cell Biology, Cell Cycle, Metabolism
Images & Validation
−Item 1 of 10
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | IHC-F=1:200-800, IHC-P=1:200-800, IF=1:200-800, WB=1:1000-5000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 4D2 |
| Immunogen | A synthesized peptide derived from human Caspase 3 (176-277/277aa) |
| Target | CASP3 |
| Molecular Weight | 12/29/32 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.
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25 ug total protein per lane of various lysates probed with Caspase-3 monoclonal antibody, unconjugated (orb2563024) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates probed with Caspase-3 monoclonal antibody, unconjugated (orb2563024) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (orb2563024) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Quick Database Links
Gene Symbol
CASP3
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Caspase-3 Recombinant Rabbit Monoclonal Antibody (orb2563024)
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