CD68 Recombinant Rabbit Monoclonal Antibody

SKU: orb1183943

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Description

Research Area

Innate Immunity, Myeloid Cells, SARS /COVID-19

Images & Validation

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Item 1 of 7

Tested Applications	FC, IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:500-1000, IHC-F=1:500-1000, IF=1:500-1000, Flow-Cyt=1ug/Test
Reactivity	Human

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	3G6
Immunogen	KLH conjugated synthetic peptide derived from human CD68
Target	CD68
Molecular Weight	37 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236; GP110; Macrophage antigen CD68(microsialin); macrosialin; SCARD1; Scavenger receptor class D member 1; macrosialin isoform A precursor; CD68_MOUSE; Macrosialin; Gp110; scavenger receptor class D, member 1; macrophage antigen CD68; LAMP4.

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CD68 Recombinant Rabbit Monoclonal Antibody

4% Paraformaldehyde-fixed THP-1 (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (CD68) monoclonal Antibody, unconjugated (orb1183943) 1:100, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei.

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CD68 antibody (orb1183943), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (orb1183943) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (orb1183943) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (orb1183943) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (orb1183943) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (orb1183943) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CD68

UniProt

P34810

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence