Cholesterol (animal-free)

SKU: orb1310316

Description

Cholesterol is a fundamental mammalian sterol constituting 20–25% of the plasma membrane, where it modulates fluidity and permeability. As an endogenous ERRα agonist and precursor for hormones and bile acids, it is integral to metabolic studies and is commonly employed in hyperlipidemia research models, both in vitro and in vivo.

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research

Images & Validation

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Item 1 of 1

Key Properties

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CAS Number	57-88-5
MW	386.664
Purity	99.94% (May vary between batches)
Formula	C₂₇H₄₆O
SMILES	CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C
Target	ROR\|\|\|Estrogen Receptor/ERR\|\|\|Endogenous Metabolite\|\|\|MRP
Solubility	H2O:< 1 mg/mL (insoluble);Ethanol:11.42 mg/mL (29.54 mM);DMSO:< 1mg/mL (insoluble)

Bioactivity

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In Vivo

METHODS: To induce hypercholesterolemia, STD:ddY mice were fed a high cholesterol diet (1% cholesterol, 0.5% cholic acid, 0.5% olive oil and 93% standard mouse chow). RESULTS: Cholesterol can be used to construct a mouse model of hypercholesterolemia. METHODS: To induce hyperlipidemia, CD-1 mice were fed a high cholesterol diet (2% cholesterol and 0.6% sodium deoxycholate). RESULTS: Cholesterol can be used to construct a mouse model of hyperlipidemia.

In Vitro

METHODS: CD4+ T lymphocytes were incubated with 7-KC (17.5-70 µM) and Cholesterol-MβCD (17.5-70 µM) for 10 min, and T cell membrane order and disorder were assessed using di-4 ANEPPDHQ fluorescent dye. RESULTS: After exposure to 7-KC, T cell membrane order was altered in a dose-dependent manner, with significant reconstitution of membrane order observed only in cells treated with 35 µM Cholesterol, while reconstitution with l7.5 µM Cholesterol induced minimal effects. METHODS: Human gastric cancer cells SNU601, SNU638 and SNU216 were treated with Cholesterol (25-100 µM) for 48 h and cell viability was measured using MTT Assay. RESULTS: Cholesterol caused a dose-dependent decrease in cell viability in all three cell lines.

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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signaling, phosphorylation, RORα, SREBP2, transporters, unesterified, inhibit, MRP1, IRS-1, insulin, Inhibitor, Endogenous Metabolite, EndogenousMetabolite, exocytic, ERR, ERRα, Estrogen Receptor, EstrogenReceptor, Estrogen Receptor/ERR, Diabetes, Cholesteryl alcohol, Cholesterin, Cholesterol, AKT

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Compound Identifiers

PubChem CID

304 ↗

Key Properties

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No computed properties available.

Protocol Information

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Cholesterol (animal-free)

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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