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Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody
SKU: orb559074
Featured
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 6
| Tested Applications | ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:1000-5000, IHC-P=1:500-2000, IHC-F=1:200-1000, ICC/IF=1:100-500, IF=1:200-1000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 2H5 |
| Immunogen | A synthesized peptide derived from human Cytokeratin 5 (550-590aa) |
| Target | KRT5 |
| Molecular Weight | 64 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Active () Monoclonal Antibody Primary Antibody Unconjugated Rabbit Cytokeratin 5 Unmodified "WB(1:1000-2000)ICC(1:50-200); IHC(1:50-200); "
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Paraformaldehyde-fixed, paraffin embedded (Human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 5) Monoclonal Antibody, Unconjugated (orb559074) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse trachea), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: human A431 cell lysates, Primary: Anti-Cytokeratin 5 (orb559074) at 1/500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1/5000 dilution, Predicted band size: 64 kD, Observed band size: 62 kD.
Quick Database Links
Gene Symbol
KRT5
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody (orb559074)
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