MPO-IN-28

SKU: orb1301873

Description

MPO-IN-28 is a potent and irreversible inhibitor of Myeloperoxidase (MPO), exhibiting an IC50 value of 44 nM. This small molecule is a valuable research tool for investigating the role of MPO in inflammatory and cardiovascular diseases, supporting both in vitro biochemical assays and in vivo experimental models.

Research Area

Metabolism Research

Images & Validation

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Key Properties

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CAS Number	37836-90-1
MW	231.25
Purity	99.02%
Formula	C₁₁H₁₃N₅O
SMILES	COc1ccc2c(C)nc(NC(N)=N)nc2c1
Target	Glutathione Peroxidase
Solubility	10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (8.65 mM);DMSO:22.73 mg/mL (98.29 mM)

Bioactivity

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Target IC50	MPO:44 nM (cell free)
In Vitro	5 μM of MPO-IN-28 was added to MPO in the presence of 30 μM of guaiacol. in the absence of H2O2, there was no Inhibition of MPO, clearly underlining that MPO-IN-28 is an irreversible mechanism-based inhibitor. MPO-IN-28 inhibit the growth of normal human dermal fibroblast (NHDF) at a concentration IC50 of 17 μM, which is about 400 times higher tha the concentration o the MPO inhibitory effect (0.044 μM). Read more...
Cell Research	To evaluate whether selected compounds exhibited toxicity a the cellular level at concentration ranging from 0.005 to 50 μM the colorimetric assay MTT was performed58 in normal human dermal fibroblasts (NHDF). Briefly, cell line was cultured in cell culture flasks, grown, and maintained at 37℃, 95% humidity, 5% CO2 in fibroblast medium FBM supplemented with 2% fetal bovine serum, 0.1% insulin, rhFGF-B, and gentamicin and amphotericin. NHDF cells were chemically detached with trypsin and seeded in 96-well plates and left to attach for 24 h. Prior to treatment, compounds were dissolved in DMSO at a concentration of 10 mM, and cells were treated wit the different concentration o the compounds diluted in culture medium (5 nM to 50 μM) or left untreated for 72 h the amount of viable cells was determined throug the MTT mitochondrial reduction into formazan by living cells according to previously described.5 the optical density (OD) was measured in a Biorad 680RX plate reader at 570 nM (reference 630 nM), an the OD o the untreated control was normalized as 100% of viable cells, allowing determination o the concentration that inhibited their growth by 50% IC50 . Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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inhibit, Myeloperoxidase, MPO IN 28, MPO, MPOIN28, MPO-IN-28, GlutathionePeroxidase, Glutathione Peroxidase, Inhibitor

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Compound Identifiers

PubChem CID

2835211 ↗

Key Properties

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No computed properties available.

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MPO-IN-28 (orb1301873)

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Purity:	>98% (HPLC)
CAS Number:	37836-90-1
MW:	231.3
Formula:	C₁₁H₁₃N₅O

MPO-IN-28

Description

Research Area

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Key Properties

Bioactivity

Storage & Handling

Alternative Names

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