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Description
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, WB |
|---|---|
| Dilution Range | WB: 1:8000, DB: 1:500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1016 of human EGFR. Antigen Region: 1000-1023 aa. |
| Target | EGFR (HGNC:3236) |
| Molecular Weight | 134277 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Similar Products
−- Item 1 of 2
Phospho-EGFR(Y1016) Antibody [orb1931130]
DOT, WB
Mouse
Human
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl
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Western blot analysis of extracts from A431 cells, untreated or treated with EGF, 100ng/ml, using EGFR Antibody (pY1016) (upper) or Beta-actin (lower).
Dot blot analysis of anti-EGFR B1 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Quick Database Links
Gene Symbol
EGFR (HGNC:3236)
UniProt
RefSeq (Protein):NP_958441.1, NP_958439.1, NP_005219.2, NP_958440.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_958441.1, NP_958439.1, NP_005219.2, NP_958440.1 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
DOT
Dot Blot
Phospho-EGFR(Y1016) Antibody (orb1788335)
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