Evo Super M-MLV Plus 1st Strand cDNA Synthesis Mix

This product is derived from total RNA or poly(A).＋ A kit for the initial synthesis of 1st strand cDNA from RNA. Reverse transcriptase for 1st strand cDNA synthesis Evo Super M-MLV The plus, RNase Inhibitor, Oligo dTPrimer, dNTP Mixture and Reaction Buffer have been premixed into a Premix-type reagent, which is easy and fast to start the reaction by adding RNA template and water. By adding auxiliary proteins and optimizing the Buffer composition, this product reduces the RNA denaturation operation before the reverse transcription reaction and makes the operation more convenient. This product is characterized by high synthesis efficiency, fast reaction speed, high sensitivity, high thermal stability and strong resistance to inhibition, etc. It is able to synthesize 1st Strand cDNA more efficiently under the condition of 42℃~60℃; meanwhile, since the reverse transcription reaction can be carried out at high temperature, the synthesis of 1st Strand cDNA by using the Gene Specific Primer enhances the efficiency and specificity of reverse transcription. and specificity of 1st Strand cDNA synthesized with Gene Specific Primer. The 1st strand cDNA synthesized in this product is suitable for 2nd strand cDNA synthesis, hybridization, PCR amplification, and preparation of full-length cDNA libraries. This product contains Random 6 mers, which can be used for the preparation of full-length cDNA libraries from poly(A)＋ It is also suitable for Real Time PCR for gene expression analysis.