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Human Interferon Gamma (IFNg) EasyStep ELISA Kit

SKU: orb2302965
Free Kit Promotion

Description

The Human Interferon Gamma (IFNg) EasyStep ELISA Kit uses a sandwich enzyme immunoassay to quantify Interferon Gamma (IFNg) EasyStep in human serum, plasma, cell. The kit has a detection range of 15.63-1000 pg/mL and a sensitivity of 15 pg/mL.

Research Area

Infectious Disease & Virology; Immunology & Inflammation

Images & Validation

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Human Interferon Gamma (IFNg) EasyStep ELISA Kit
Application Notes
standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ, and the Human IFN-γ standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IFN-γ. After TMB substrate solution is added, only those wells that contain Human IFN-γ and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFN-γ in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityHuman
Sample TypesSerum, plasma, cell
Assay TypeSandwich
Assay Time1.5h
Range15.63-1000 pg/mL
Sensitivity15 pg/mL
Concentration1000 pg/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

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48 T
$ 330.00
96 T
$ 470.00
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