Human S100 Calcium Binding Protein B (S100B) ELISA Kit

SKU: orb776476

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Description

The Human S100 Calcium Binding Protein B (S100B) ELISA Kit uses a sandwich enzyme immunoassay to quantify S100 Calcium Binding Protein B (S100B) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.058 ng/mL.

Research Area

Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S100B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.058 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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S100-B ELISA Kit, NEF ELISA Kit, S100Beta ELISA Kit, S-100 protein subunit beta ELISA Kit, S100 protein beta chain ELISA Kit, Soluble protein-100B ELISA Kit, Soluble protein 100B ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt

P04271

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (1)

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Human (1)

NIU Lin-jie, T Meng, Y Ruo-lin… - Journal of Hebei Study on the impact of cerebral oxygen saturation on postoperative cognitive dysfunction and inflammatory response in elderly patients undergoing hip surgery during xuebao.hebmu.edu.cn, (2024)

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Applications

ELISA

Reactivity

Human

Reactivity:	Human
Dynamic Range:	31.25-2000pg/mL
Sensitivity:	13.7 pg/mL

Reactivity:	Human
Dynamic Range:	31.25-2000pg/mL
Sensitivity:	18.75 pg/mL

Reactivity:	Human
Dynamic Range:	0.16-10 ng/mL
Sensitivity:	0.059 ng/mL

Reactivity:	Human
Dynamic Range:	31.25-2000pg/ml
Sensitivity:	18.75pg/ml

Reactivity:	Human
Dynamic Range:	7.81-500pg/mL
Sensitivity:	3.67pg/mL

Human S100 Calcium Binding Protein B (S100B) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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