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Human Thyroid Transcription Factor 1 (TITF1) ELISA Kit

SKU: orb778311
Free Kit Promotion

Description

The Human Thyroid Transcription Factor 1 (TITF1) ELISA Kit uses a sandwich enzyme immunoassay to quantify Thyroid Transcription Factor 1 (TITF1) in human tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.057 ng/mL.

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA
Application Notes
standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TITF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TITF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TITF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TITF1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

ReactivityHuman
Sample Typestissue homogenates, cell lysates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.057 ng/mL
Concentration10 ng/mL

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

TTF1 ELISA Kit, NKX2-1 ELISA Kit, BCH ELISA Kit, BHC ELISA Kit, NK-2 ELISA Kit, NKX2.1 ELISA Kit, NKX2A ELISA Kit, TEBP ELISA Kit, NK2 Homeobox 1 ELISA Kit, Benign Chorea ELISA Kit, Thyroid nuclear factor 1 ELISA Kit, Thyroid-specific enhancer-binding protein ELISA Kit

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
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