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Mouse Amphiregulin (AREG) ELISA Kit

SKU: orb774868
Free Kit Promotion

Description

The Mouse Amphiregulin (AREG) ELISA Kit uses a sandwich enzyme immunoassay to quantify Amphiregulin (AREG) in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 15.63-1000 pg/mL and a sensitivity of 6.1 pg/mL.

Research Area

Cancer Biology

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AREG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AREG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AREG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AREG in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityMouse
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range15.63-1000 pg/mL
Sensitivity6.1 pg/mL
Concentration1000 pg/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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AR ELISA Kit, CRDGF ELISA Kit, SDGF ELISA Kit, Colorectum Cell-Derived Growth Factor ELISA Kit, Schwannoma-Derived Growth Factor ELISA Kit

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

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No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

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Bingsheng Yang # 1 2 3, Jianwen Su # 1 2, Jichang Wu # 1 2, Zhongwen Wang 1 2, Jin Hu 1 2, Mankai Yang 1 2, Yihuang Lin 1 2, Mingchao Jin 1 2, Xiaochun Bai 4, Bin Yu 1 2, Xianrong Zhang 5 6 Macrophage-derived amphiregulin induces myofibroblast transition in adipogenic lineage precursors near Staphylococcus aureus abscess in bone marrow Nat Commun, (2025)

Applications

ELISA

Reactivity

Mouse

Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
DispatchUsually dispatched within 5-10 working days
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