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Mouse IL-12p40(Interleukin 12 p40) Microsample ELISA Kit

SKU: orb2998789
Free Kit Promotion

Description

The Mouse IL-12p40(Interleukin 12 p40) Microsample ELISA Kit is designed to quantify IL-12p40(Interleukin 12 p40) Microsample in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 15.63-1000 pg/mL and a sensitivity of 5.6 pg/mL.

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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ReactivityMouse
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range15.63-1000 pg/mL
Sensitivity5.6 pg/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

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No UniProt data available

Protocol Information

Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
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