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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Item 1 of 6
| Tested Applications | FC, IF, IHC, IP, WB |
|---|---|
| Dilution Range | WB 1:1000, IP: 20 µl/mg lysate, IHC: 1:100 - 1:500, Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cell. mIF: 1:250 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BL-298-5D12 |
| Immunogen | Between 157 and C-terminus |
| Target | CD3E |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD antigen CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; IMD18; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000724.1 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
IP
Immunoprecipitation
Available Sizes
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