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Description
Research Area
Immunology & Inflammation
Images & Validation
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| Application Notes |
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Key Properties
−| Source | Escherichia coli. |
|---|---|
| Biological Activity | Fully biologically active when compared to standard.Measured by its ability to induce STAT3 activation in U-87 MG human glioblastoma/astrocytoma cells. 5 ng/mL of rhIL31 can effectively induce STAT3 activation. |
| Purification | >97% by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1EU/mg of rHuIL-31 as determined by LAL method. |
| MW | Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids. |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Protein Sequence | SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T |
Storage & Handling
−| Storage | This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. |
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| Form/Appearance | Lyophilized from a 0.2mm filtered concentrated solution in PBS. |
| Buffer/Preservatives | Lyophilized from a 0.2mm filtered concentrated solution in PBS. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Similar Products
−Human Interleukin-31 (rHu IL-31) Recombinant Protein [orb1631635]
> 97 % by SDS-PAGE and HPLC analyses.
Approximately 15.8 kDa
10 μl
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide