Activin Receptor Type IIB Rabbit Polyclonal Antibody

SKU: orb155589

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Description

Research Area

Actin Binding Proteins, Cell Biology, Kinases, Molecular Biology, Surface Molecules

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Porcine, Rat, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human ACVR2B/ACTR-IIB (45-150/512aa)
Target	ACVR2B
Molecular Weight	56 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Activin Receptor Type IIB; ACTR-IIB; Activin A receptor type IIB; Activin receptor type 2B; Activin receptor type IIB; Activin receptor type-2B; ActR IIB; ACTR-IIB; ActRIIB; ACVR 2B; ACVR2B; AVR2B_HUMAN; HTX4; MGC116908.

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Activin Receptor Type IIB Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Activin Receptor Type IIB) Polyclonal Antibody, Unconjugated (orb155589) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: K562 (Human) Cell Lysate at 30 ug, 293T (Human) Cell Lysate at 30 ug, Primary: Anti-Activin Receptor Type IIB (orb155589) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Muscle tissue lysates, Lane 3: Mouse Testis tissue lysates, Lane 4: Mouse Kidney tissue lysates, Lane 5: Human K562 cell lysates, Lane 6: Human SH-SY5Y cell lysates, Lane 7: Human Jurkat cell lysates, Lane 8: Human MOLT4 cell lysates, Primary: Anti-Activin Receptor Type IIB (orb155589) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 60 kDa.

Quick Database Links

Gene Symbol

ACVR2B

UniProt

Q13705

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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