Alpha Adaptin Rabbit Polyclonal Antibody

SKU: orb10104

Featured

Description

Images & Validation

−

Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Bovine, Equine, Human, Rabbit

Related Conjugates & Formulations

−

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human alpha Adaptin (21-150/939aa)
Target	AP2A2
Molecular Weight	103 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

−

100 kDa coated vesicle protein A; 100 kDa coated vesicle protein C; adaptin alpha A; Adaptin alpha B; Adaptor protein complex AP 2 alpha 1 subunit; Adaptor protein complex AP 2 alpha 2 subunit; Adaptor related protein complex 2 alpha 1 subunit; Adaptor related protein complex 2 alpha 2 subunit; ADTAA; ADTAB; alpha adaptin A; alpha adaptin C; AP 2 complex subunit alpha 1; AP 2 complex subunit alpha 2; AP2A2; CLAPA1; CLAPA2; Clathrin assembly protein complex 2 alpha A large chain; Clathrin assembly protein complex 2 alpha C large chain; Clathrin associated/assembly/adaptor protein large alpha 1; Clathrin associated/assembly/adaptor protein large alpha 2; HIP9; Huntingtin interacting protein HYPJ; Huntingtin interacting protein J; HYPJ; KIAA0899; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit; AP2A2_HUMAN; AP-2 complex subunit alpha-2; Adapter-related protein complex 2 alpha-2 subunit; Adaptor protein complex AP-2 subunit alpha-2; alpha-adaptin C; alpha2-adaptin; Clathrin assembly protein complex 2 alpha-C large chain; Huntingtin yeast partner J; Huntingtin-interacting protein 9; HIP-9; Huntingtin-interacting protein J; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Alpha Adaptin Rabbit Polyclonal Antibody

Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-alpha Adaptin (orb10104) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 103 kD, Observed band size: 103 kD.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (alpha Adaptin) Polyclonal Antibody, Unconjugated (orb10104) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-alpha Adaptin (orb10104) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 103 kD, Observed band size: 103 kD.

Quick Database Links

Gene Symbol

AP2A2

UniProt

O94973

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

View Protocol

Immunofluorescence

View Protocol

Find More Protocols