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Alpha B Crystallin Rabbit Polyclonal Antibody

SKU: orb100298

Description

Alpha B Crystallin Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Tyrosine Kinase Signaling Pathway, Tyrosine Kinases, Visual System

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution RangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityMouse, Rat
Predicted ReactivityBovine, Canine, Equine, Human, Porcine

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Alpha B Crystallin. (51-150/175aa)
TargetCRYAB
Molecular Weight20 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CMD1II; CRYA2; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2; MFM2A; MFM2B; Crya-2; P23; AACRYA; CRYAB_HUMAN; CRYAB; Alpha(B)-crystallin; Heat shock protein beta-5 (HspB5); Heat shock protein family B member 5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component; CRYAB_MOUSE; CRYAB_RAT;

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Alpha B Crystallin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Alpha B Crystallin) Polyclonal Antibody, Unconjugated (orb100298) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Alpha B Crystallin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Alpha B Crystallin) Polyclonal Antibody, Unconjugated (orb100298) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Alpha B Crystallin Rabbit Polyclonal Antibody (orb100298)

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Available Sizes

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50 μl
¥ 3,250.00
100 μl
¥ 5,070.00
200 μl
¥ 6,110.00
Free Secondary Antibody (20 ul)0/0

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