Beclin 1/BECN1 Rabbit Polyclonal Antibody

SKU: orb196247

Description

Anti-Beclin 1/BECN1 Antibody. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Metabolism Research, Microbiology, Signal Transduction

Images & Validation

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Item 1 of 7

Tested Applications	FC, ICC, IF, IP, WB
Dilution Range	Western blot, 0.1-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human Beclin 1 recombinant protein (Position: M1-S354). Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse and rat Beclin 1.
Target	Beclin-1
Molecular Weight	60 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; BECN1; GT197

Quality Guarantee

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Beclin 1/BECN1 Rabbit Polyclonal Antibody

Flow Cytometry analysis of HepG2 cells using anti-Beclin-1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Beclin 1 using anti-Beclin 1 antibody.Lane 1:human Colo320 cell; 2:human HepG2 cell; 3:human PANC cell; 4:human A431 cell; 5:human Smmc cell; 6:human Jurkat cell; 7:human SW620 cell; 8:human U87 cell.

IF analysis of Beclin-1 using anti-Beclin-1 antibody. Beclin-1 was detected in immunocytochemical section of U20S cells.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of mouse intestine tissue.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of mouse spleen tissue.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of rat intestine tissue.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of rat spleen tissue.

Quick Database Links

Gene Symbol

Beclin-1

UniProt

Q14457

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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Immunoprecipitation

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