beta Actin/ACTB Rabbit Polyclonal Antibody

SKU: orb1804713

Description

Anti-beta Actin/ACTB Antibody. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken, Zebrafish.

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat, Chicken, Zebrafish
Reactivity	Gallus, Human, Monkey, Mouse, Rat, Zebrafish

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.
Target	Actin, cytoplasmic 1
Molecular Weight	42 kDa
Purification	The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB

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beta Actin/ACTB Rabbit Polyclonal Antibody

WB analysis of beta Actin/ACTB using anti-beta Actin/ACTB antibody.Lane 1:human HeLa cell;2:human K562 cell;3:human MCF-7 cell;4:human Jurkat cell;5:rat brain tissue;6:rat kidney tissue;7:mouse brain tissue;8:mouse kidney tissue.

WB analysis of beta Actin/ACTB using anti-beta Actin/ACTB antibody.Lane 1:zebrafish tissue;2:chicken heart tissue;3:chicken liver tissue;4:chicken brain tissue;5:monkey heart tissue;6:monkey lung tissue;7:monkey kidney tissue;8:monkey liver tissue.

Quick Database Links

Gene Symbol

Actin, cytoplasmic 1

UniProt

P60709

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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