BID Rabbit Polyclonal Antibody

SKU: orb182359

Description

Research Area

Cardiovascular Research, Immunology & Inflammation, Metabolism Research

Images & Validation

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Item 1 of 7

Tested Applications	FC, ICC, IF, IHC, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively.
Target	BH3-interacting domain death agonist
Molecular Weight	22 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Antigen KI-67; MKI67

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Flow Cytometry analysis of MCF-7 cells using anti-BID antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of BID using anti-BID antibody.Lane 1:HELA Cell;2:COLO320 Cell;3:JURKAT Cell;4:SKOV Cell.

IF analysis of Bid using anti-Bid antibody. Bid was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of human mammary cancer tissues.

IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of mouse spleen tissues.

IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of rat intestine tissues.

IHC analysis of BID using anti-BID antibody.BID was detected in immunocytochemical section of A549 cell.

Quick Database Links

Gene Symbol

BH3-interacting domain death agonist

UniProt

P55957

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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