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Ccl3 Antibody
SKU: orb1147787
Description
Research Area
Cardiovascular Research, Cell Biology, Metabolism Research, Pharmacology & Drug Discovery, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC |
|---|---|
| Dilution range | Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat ELISA, 0.1-0.5 μg/ml, - |
| Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived mouse Ccl3 recombinant protein (Position: A24-A92). |
| Target | C-C motif chemokine 3 |
| Molecular Weight | 65 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
−C C motif chemokine 3; CCL3; chemokine (C C motif) ligand 3; G0S19 1; LD78 alpha(4 69); LD78ALPHA; MIP 1 alpha; MIP1A; PAT 464.1; SCYA3; SIS beta; Small inducible cytokine A3
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IHC analysis of Ccl3 using anti-Ccl3 antibody. Ccl3 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Ccl3 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Ccl3 using anti-Ccl3 antibody. Ccl3 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Ccl3 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Quick Database Links
Gene Symbol
C-C motif chemokine 3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Ccl3 Antibody (orb1147787)
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