CD10/MME Rabbit Polyclonal Antibody

SKU: orb182390

Description

Anti-CD10/MME Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Signal Transduction

Images & Validation

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Item 1 of 5

Tested Applications	FC, IHC, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
Reactivity	Human, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human CD10 recombinant protein (Position: Y52-W750). Human CD10 shares 94% amino acid (aa) sequences identity with both mouse and rat CD10.
Target	Neprilysin
Molecular Weight	100 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Neprilysin; 3.4.24.11; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD10; MME; EPN

Quality Guarantee

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Flow Cytometry analysis of Daudi cells using anti-CD10/MME antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of CD10/MME using anti-CD10/MME antibody.Lane 1:human Daudi cell;2:human U-87MG cell;3:human placenta tissue;4:rat kidney tissue.

IHC analysis of CD10/MME using anti-CD10/MME antibody. CD10/MME was detected in a paraffin-embedded section of human lymphoma tissue.

IHC analysis of CD10/MME using anti-CD10/MME antibody. CD10/MME was detected in a paraffin-embedded section of human placenta tissue.

IHC analysis of CD10/MME using anti-CD10/MME antibody. CD10/MME was detected in a paraffin-embedded section of human renal cell carcinoma tissue.

Quick Database Links

Gene Symbol

Neprilysin

UniProt

P08473

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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