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Cd4 Rabbit Polyclonal Antibody

SKU: orb1098034

Description

Anti-Cd4 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Rat.

Research Area

Cell Biology, Neuroscience, Stem Cell & Developmental Biology

Images & Validation

Tested ApplicationsELISA, IHC
Dilution RangeImmunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat ELISA, 0.1-0.5 μg/ml, Rat
ReactivityRat

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE.coli-derived rat Cd4 recombinant protein (Position: K28-A246).
TargetNatriuretic peptide A
Molecular Weight52 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD4; CD4 molecule; CD4mut

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Cd4 Rabbit Polyclonal Antibody

IHC analysis of Cd4 using anti-Cd4 antibody. Cd4 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cd4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

UniProt Details

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Cd4 Rabbit Polyclonal Antibody (orb1098034)

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Available Sizes

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100 μg
¥ 5,850.00
DispatchUsually dispatched within 2-4 weeks
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