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Cofilin/CFL1 Rabbit Polyclonal Antibody
SKU: orb182365
Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 9
| Tested Applications | ELISA, FC, ICC, IF, IHC, IHC-Fr, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human ELISA, 0.1-0.5μg/ml, - |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human Cofilin recombinant protein (Position: A2-L166). Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin. |
| Target | Cofilin-1 |
| Molecular Weight | 19 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform; CFL1; CFL
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Quality Guarantee
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Flow Cytometry analysis of U20S cells using anti-Cofilin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Cofilin using anti-Cofilin antibody.Lane 1:human HeLa cell;2:monkey COS-7 cell;3:human HEK293 cell;4:human U-87MG cell;5:human HepG2 cell;6:human PC-3 cell;7:human THP-1 cell;8:human A549 cell.
WB analysis of Cofilin using anti-Cofilin antibody.Lane 1:rat heart tissue;2:rat spleen tissue;3:rat lung tissue;4:rat C6 cell;5:mouse spleen tissue;6:mouse NIH/3T3 cell;7:mouse HEPA1-6 cell.
IF analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in immunocytochemical section of U20S cell.
IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in paraffin-embedded section of mouse lung tissues.
IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in paraffin-embedded section of rat lung tissues.
IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in paraffin-embedded section of human intestinal cancer tissues.
IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in paraffin-embedded section of human mammary cancer tissues.
IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in frozen section of human placenta tissues.
Quick Database Links
Gene Symbol
Cofilin-1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Cofilin/CFL1 Rabbit Polyclonal Antibody (orb182365)
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