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DEP1/PTPRJ Rabbit Polyclonal Antibody

SKU: orb1289989

Description

Anti-DEP1/PTPRJ Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Rat.

Research Area

Cell Biology, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC, WB
Dilution RangeWestern blot, 0.25-0.5 μg/ml, Human, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
ReactivityHuman, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE.coli-derived human DEP1/PTPRJ recombinant protein (Position: D143-E891).
TargetReceptor-type tyrosine-protein phosphatase eta
Molecular Weight240 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD148; Density enhanced phosphatase 1; DEP 1; DEP1; HPTP eta; HPTPeta; PTPRJ; R PTP ETA; R PTP J; SCC1

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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FC
Flow Cytometry
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IF
Immunofluorescence
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Zhan Zhang 1, Lili Chen 2, Qiao Yang 3, Xiaowan Tang 3, Jianhua Li 3, Guangwen Zhang 3, Youqun Wang 3, Hui Huang INHBA regulates Hippo signaling to confer 5-FU chemoresistance mediated by cellular senescence in colon cancer cells Int J Biochem Cell Biol, (2024)

Applications

IHC

Reactivity

Human

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-4 weeks
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