Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2b
Clone No.	19b10
Immunogen	A synthetic peptide corresponding to a sequence of His Tag.
Target	Guanine nucleotide-binding protein G
Molecular Weight	12 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration	500 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ATP synthase D chain mitochondrial antibody; ATP synthase H+ transporting mitochondrial F1F0 subunit antibody; ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody; ATP synthase subunit d antibody; ATP synthase subunit d, mitochondrial antibody; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody; ATP5H antibody; ATP5H_HUMAN antibody; ATP5JD antibody; ATPase subunit d antibody; ATPQ antibody; mitochondrial antibody; My032 protein antibody

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Western blot analysis of His Tag using anti-His Tag antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse His Tag protein 10 ng, Lane 1: recombinant mouse His Tag protein 5 ng, Lane 1: recombinant mouse His Tag protein 2.5 ng, Lane 1: recombinant mouse His Tag protein 1.25 ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-His Tag antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

Quick Database Links

Gene Symbol

Guanine nucleotide-binding protein G

UniProt

P50150

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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