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Huntingtin/HTT Rabbit Polyclonal Antibody

SKU: orb1819351

Description

Anti-Huntingtin/HTT Antibody. Tested in ELISA, IHC applications. This antibody reacts with Human.

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC
Dilution RangeImmunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, -
ReactivityHuman

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenE.coli-derived human Huntingtin/HTT recombinant protein (Position: R3079-C3142). Human HTT shares 93.8% and 92.2% amino acid (aa) sequence identity with mouse and rat HTT, respectively.
TargetHuntingtin
Molecular Weight65 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

HD; HD protein; HTT; huntingtin; Huntington disease protein; IT15

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Huntingtin/HTT Rabbit Polyclonal Antibody

IHC analysis of Huntingtin/HTT using anti-Huntingtin/HTT antibody. Huntingtin/HTT was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Huntingtin/HTT Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Huntingtin/HTT Rabbit Polyclonal Antibody

IHC analysis of Huntingtin/HTT using anti-Huntingtin/HTT antibody. Huntingtin/HTT was detected in a paraffin-embedded section of human cervix squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Huntingtin/HTT Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Huntingtin/HTT Rabbit Polyclonal Antibody

IHC analysis of Huntingtin/HTT using anti-Huntingtin/HTT antibody. Huntingtin/HTT was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Huntingtin/HTT Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Huntingtin/HTT Rabbit Polyclonal Antibody

IHC analysis of Huntingtin/HTT using anti-Huntingtin/HTT antibody. Huntingtin/HTT was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Huntingtin/HTT Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Huntingtin/HTT Rabbit Polyclonal Antibody

IHC analysis of Huntingtin/HTT using anti-Huntingtin/HTT antibody. Huntingtin/HTT was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Huntingtin/HTT Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Huntingtin/HTT Rabbit Polyclonal Antibody

IHC analysis of Huntingtin/HTT using anti-Huntingtin/HTT antibody. Huntingtin/HTT was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Huntingtin/HTT Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Huntingtin/HTT Rabbit Polyclonal Antibody

IHC analysis of Huntingtin/HTT using anti-Huntingtin/HTT antibody. Huntingtin/HTT was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Huntingtin/HTT Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

UniProt Details

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Protocol Information

IHC
Immunohistochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Huntingtin/HTT Rabbit Polyclonal Antibody (orb1819351)

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100 μg
¥ 5,850.00
DispatchUsually dispatched within 2-4 weeks
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