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ICAM1 Rabbit Polyclonal Antibody
SKU: orb180715
Description
Research Area
Cardiovascular Research, Cell Biology, Disease Biomarkers, Immunology & Inflammation, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 5
| Tested Applications | FC, ICC, IF, IHC, IHC-Fr, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human ICAM1 recombinant protein (Position: L214-P532). Human ICAM1 shares 52% and 48% amino acid (aa) sequences identity with mouse and rat ICAM1, respectively. |
| Target | Intercellular adhesion molecule 1 |
| Molecular Weight | 100 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54; ICAM1
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Flow Cytometry analysis of A431 cells using anti-ICAM1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of ICAM1 using anti-ICAM1 antibody.Lane 1:HEPG2 Cell;2:K562 Cell;3:A549 Cell;4:HT1080 Cell.
IF analysis of ICAM1 using anti-ICAM1 antibody.ICAM1 was detected in paraffin-embedded section of human tonsil tissues.
IF analysis of ICAM1 using anti-ICAM1 antibody.ICAM1 was detected in paraffin-embedded section of human Lung cancer tissues.
IHC analysis of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in paraffin-embedded section of human intestinal cancer tissues.
Quick Database Links
Gene Symbol
Intercellular adhesion molecule 1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ICAM1 Rabbit Polyclonal Antibody (orb180715)
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