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IGFBP2 Antibody
SKU: orb312131
Description
Research Area
Cancer Biology, Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Dilution range | Western blot, 0.1-0.5μg/ml, Human, Rat |
| Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human IGFBP2, different from the related mouse and rat sequences by one amino acid. |
| Target | Insulin-like growth factor-binding protein 2 |
| Molecular Weight | 35 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
−Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2; IGFBP2; BP2, IBP2
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Western blot analysis of IGFBP2 using anti-IGFBP2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50 ug, Lane 2: Rat Liver Tissue Lysate at 50 ug, Lane 3: Human Placenta Tissue Lysate at 50 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP2 at approximately 35 kDa. The expected band size for IGFBP2 is at 35 kDa.
Quick Database Links
Gene Symbol
Insulin-like growth factor-binding protein 2
UniProt
UniProt Details
− No UniProt data available
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IGFBP2 Antibody (orb312131)
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