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IL-10/IL10 Antibody

SKU: orb1184717

Description

Anti-IL-10/IL10 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Human.

Images & Validation

Tested ApplicationsELISA, IHC
ReactivityHuman
Application Notes
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human ELISA, 0.1-0.5 μg/ml/ml, Human. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE.coli-derived human IL-10/IL10 recombinant protein (Position: K137-N178).
TargetReceptor tyrosine-protein kinase erbB-2
Molecular Weight19 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
DisclaimerFor research use only

Alternative Names

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10

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IL-10/IL10 Antibody

IHC analysis of IL-10/IL10 using anti-IL-10/IL10 antibody. IL-10/IL10 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-10/IL10 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

UniProt Details

No UniProt data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IL-10/IL10 Antibody (orb1184717)

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Available Sizes

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100 μg
¥ 5,850.00
DispatchUsually dispatched within 2-4 weeks
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