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Description
Research Area
Cell Biology, Epigenetics & Chromatin, Immunology & Inflammation, Neuroscience
Images & Validation
−
Item 1 of 4
| Tested Applications | ELISA, FC, IHC, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.25μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Mouse ELISA, 0.1-0.5μg/ml |
| Reactivity | Human, Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived mouse Nefh recombinant protein (Position: Y109-E466). |
| Target | Neurofilament heavy polypeptide |
| Molecular Weight | 117-220 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4. |
| Concentration | 500 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NEFH; NEH; NF; NF H; NF200; NF-H; Neurofilament H
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Quick Database Links
Gene Symbol
Neurofilament heavy polypeptide
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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