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Description
Research Area
Cancer Biology, Epigenetics & Chromatin, Metabolism Research, Signal Transduction
Images & Validation
−
Item 1 of 8
| Tested Applications | FC, ICC, IF, IHC, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells |
| Reactivity | Human, Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human NSE, which shares 95.1% and 100% amino acid (aa) sequence identity with mouse and rat NSE, respectively. |
| Target | Gamma-enolase |
| Molecular Weight | 47 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−enolase 2 (gamma; neuronal); Gamma enolase; Neural enolase; Neuron specific enolase; NSE
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Quick Database Links
Gene Symbol
Gamma-enolase
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Filter by Applications
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Valeria Manzini 1 2, Pamela Cappelletti 3, Nicola S Orefice 4 5 6, Ilaria Brentari 7, Michael J Rigby 4 5 8 9, Maria Lo Giudice 10, Marco Feligioni 3 11, Roberto Rivabene 1, Alessio Crestini 1, Francesco Manfredi 12, Giuseppina Talarico 13, Giuseppe Bruno 13, Massimo Corbo 3, Luigi Puglielli 4 5 14, Michela A Denti 15, Paola Piscopo miR-92a-3p and miR-320a are Upregulated in Plasma Neuron-Derived Extracellular Vesicles of Patients with Frontotemporal Dementia Mol Neurobiol, (2024)
Applications
WB
Reactivity
Human
Available Sizes
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