TRPV5 Antibody

Western blot analysis of TRPV5 using anti-TRPV5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Pancreas Tissue Lysate at 50 ug, Lane 2: Rat Lung Tissue Lysate at 50 ug, Lane 3: Rat Intestine Tissue Lysate at 50 ug, Lane 4: SW620 Whole Cell Lysate at 40 ug, Lane 5: COLO320 Whole Cell Lysate at 40 ug, Lane 6: 293T Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRPV5 at approximately 83 kDa. The expected band size for TRPV5 is at 83 kDa.