VEGF/VEGFA Rabbit Polyclonal Antibody

SKU: orb196242

Description

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 5

Tested Applications	FC, IHC, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human VEGF recombinant protein (Position: A27-R191). Human VEGF shares 78% amino acid (aa) sequence identity with both mouse and rat VEGF.
Target	Gap junction protein
Molecular Weight	18, 27 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF

Quality Guarantee

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Flow Cytometry analysis of SiHa cells using anti-VEGF antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of VEGF using anti-VEGF antibody.Lane 1:rat lung Tissue;2:rat lver Tissue;3:rat thymus Tissue;4:mouse lung Tissue;5:mouse thymus Tissue;6:HEPA1-6 Cell;7:human placenta Tissue;8:A431 Cell.

IHC analysis of VEGF using anti-VEGF antibody. VEGF was detected in paraffin-embedded section of human rectal cancer tissue.

IHC analysis of VEGF using anti-VEGF antibody. VEGF was detected in paraffin-embedded section of rat brain tissue.

Quick Database Links

Gene Symbol

Gap junction protein

UniProt

P15692

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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