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ATF4 Rabbit Polyclonal Antibody
SKU: orb576180
Description
Images & Validation
−Item 1 of 6
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of mouse ATF4 |
| Target | ATF4 |
| Molecular Weight | 42kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Atf-, C/AT, CREB, Atf-4, C/ATF, CREB2, CREB-2, TAXREB, TAXREB67
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Sample Tissue: Mouse Brain, Antibody Dilution: 1 ug/ml.
Sample Tissue: Mouse Brain, Antibody Dilution: 1 ug/ml.
Sample Tissue: Mouse Skeletal Muscle, Antibody Dilution: 3 ug/ml.
Immunohistochemistry with Human Braun, cerebellum tissue at an antibody concentration of 5.0 ug/ml using anti-ATF4 antibody (orb576180).
Rabbit Anti-ATF4 Antibody, Catalog Number: orb576180, Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow Tissue, Observed Staining: Cytoplasm, Nucleus, Primary Antibody Concentration: 1:600, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.
WB Suggested Anti-ATF4 Antibody Titration: 0.125 ug/ml, ELISA Titer: 1:62500, Positive Control: NIH/3T3 cell lysate.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ATF4 Rabbit Polyclonal Antibody (orb576180)
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