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ATP5F1B Rabbit Polyclonal Antibody
SKU: orb580321
Description
Research Area
Cell Biology, Immunology & Inflammation, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 6
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Porcine, Rabbit, Yeast, Zebrafish |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human ATP5B |
| Target | ATP5F1B |
| Molecular Weight | 57 kDa |
| Purification | Protein A purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 1.0 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−ATP5B, ATPMB, ATPSB, HEL-S-271
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 2.5 ug/ml of the antibody was used in this experiment.
ATP5B antibody - N-terminal region (orb580321) validated by WB using HepG2 cell lysate at 1.25 ug/ml.
ATP5B antibody - N-terminal region (orb580321) validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.
Sample Tissue: Mouse Kidney, Antibody dilution: 1 ug/ml.
Sample Tissue: Mouse Kidney, Antibody dilution: 1 ug/ml.
Human Muscle
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ATP5F1B Rabbit Polyclonal Antibody (orb580321)
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