BMPR1A Rabbit Polyclonal Antibody

SKU: orb10197

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Description

Research Area

Cardiovascular Research, Kinases, Molecular Biology, Stem Cell, Surface Molecules, Vitamins and Minerals

Images & Validation

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Item 1 of 6

Tested Applications	FC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Reactivity	Human, Rat
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human BMPR-1A (101-200/532aa)
Target	BMPR1A
Molecular Weight	60 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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BMPR-1A; Activin A receptor type II like kinase 3; Activin receptor like kinase 3; ACVRLK 3; ACVRLK3; ALK 3; ALK3; BMPR 1A; Bmpr; Bone morphogenetic protein receptor type IA; Bone morphogenetic protein receptor type IA precursor; BR 1a; BR1a; CD 292; CD292; CD292 antigen; EC 2.7.11.30; Serine threonine protein kinase receptor R5; Serine threonine protein kinase receptor R5 precursor; SKR 5; SKR5; zBMPR IA; zBMPRIA.

Quality Guarantee

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Blank control: A549. Primary Antibody (green line): Rabbit Anti-BMPR1A antibody (orb10197), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (orb10197) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (orb10197) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat bone), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (orb10197) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (orb10197) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human 293T cell lysates, Lane 2: Human DU145 cell lysates, Lane 3: Human HepG2 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human U251 cell lysates, Lane 6: Human A549 cell lysates, Primary: Anti-BMPR1A (orb10197) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 60 kDa.

Quick Database Links

Gene Symbol

BMPR1A

UniProt

P36894

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence